Category Archives: Technical

Ramanomics: Quantification of Proteins, Nucleic Acids, and Lipids Inside Eukaryotic Cells

Molecular function in eukaryotic cells can be studied by quantifying proteins, nucleic acids, and lipids inside intracellular organelles. Traditionally, this quantification is performed through techniques that are given “-omic” terms such as proteomics, metabolomics, lipidomics, etc. using mass spectrometry (MS)-based techniques. 

Raman spectroscopy has traditionally been used to quantify the molecular structure of a wide range of chemical species with size ranges from near-field (<100 nm) to bulk measurements. Extensive work has gone into the application of Raman spectroscopy in many biochemical applications but successful solutions have often been difficult due to the complexity of the information obtained in the measurement and interferences arising from the laser-based methods used for Raman spectroscopy. Dr. Andrey Kuzmin of the University at Buffalo and Advanced Cytometry Instrumentation Systems, LLC, has successfully bridged this gap with the application of Raman spectroscopy to the quantification of intracellular components.  His development, termed “Ramanomics” can be used to quantify proteins, DNA, RNA, and lipids in live cells.

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Anisotropic Thermal Conductivity and Diffusivity Measurements of Li-ion Batteries

Li-ion batteries represent many of the most critical areas of development in energy storage.  The appeal of these systems arise from their higher energy densities, longer life cycles (charge and discharge), and lighter weight compared to typical battery chemistries.

To read more about our ground breaking instruments for thermal testing read the following Steve’s Solutions article.

Installation Highlights

We are glad to share the news about the implementation of Barnett Technical Services installation of a significant engineering project.

The company has received the request and later an order from the Chevron energy corporation’s research center to develop an IR Fourier Spectrometer for controlling the chemical reaction in the on-line process.

The Barnet Technical Services Team along with Ostec engineers have implemented developing the industrial version on the basis of the standard air based FTIR spectrometer IROS 05. To transform the research device into an industrial unit it was necessary to develop a sealed device case filled with dry nitrogen under extra pressure, to select and replace a typical IR detector with an MCT detector with Peltier cooling, to develop a cooling system and temperature stabilization for ensure the lowest possible noise level inside the device, as well as specialized software for obtaining data and its subsequent processing.

Microplastics Isolation and Characterization

Microplastics contaminate marine, freshwater and terrestrial ecosystems around the world. The growing prevalence of these contaminants requires study on their impact on human health and ways in which they can be identified and remediated.

Barnett Technical Services (BTS) has participated in a study set up by the State of California to assess methods for counting and characterizing micro plastic particles in water. This summary illustrates some of the methods BTS used in this study.

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Failure analysis and particle isolation in a post-COVID-19 world.

The spread of COVID-19 has led to great changes in the lives of people worldwide.  This includes the need for increased social distancing and the use of Personal Protective Equipment (PPE) to minimize the risk of spreading and acquiring disease from others and the surrounding environment. The use of PPE, including gloves, safety goggles, and a mask causes difficulties for failure analysis and particle isolation where precise microscopic sample handling is often required.  Precise manipulations using probes and other handheld tools while looking through a microscope, are much more difficult while wearing PPE.

The Micro Support Axis Pro micromanipulator is an excellent solution for performing precise microscopic sampling where PPE is required.  

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EX SITU LIFT-OUT WITH A BENCHTOP MICROMANIPULATOR

Transmission Electron Microscopy (TEM) represents one of the most powerful super-microscopy techniques for material characterization. In order to obtain powerful images, it is critical to have a very thin section of material that allows for electron transmission through the sample.

The Micro Support Axis Pro micromanipulator can transfer FIB foils to TEM grids outside (ex situ) the SEM chamber. 

This article inlcudes videos, diagrams and descriptions of Lift-Out on the Micromanipulator. Click here for the entire article.

SciX 2020 virtual eXchange

We are excited to be showing an e-poster at the SciX 2020 virtual conference. The poster touches on the work we have been doing with micro plastic research instrumentation and procedure. The poster will be available at the show October 12-15 2020. We will also post it here on the site after the show.

The instruments that we discuss are the Axis Pro micromanipulator for particle isolation and the Ostec e200 Raman microscope for characterization.

Link to SciX 2020 Website
Link to e-Poster