Confocal Detection Principles
Confocal Laser Scanning Raman Microscope has become a widely recognized research instrument in recent years. Confocal microscopy offers several advantages over conventional wide-field optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane and the capability to collect serial optical sections from thick samples. The image of the extended sample is generated by scanning the focused laser beam across a defined area.
Basic Confocal Raman Microscope
- Versatile automated confocal Raman microscope
- High throughput true confocal design enabling submicron resolution
- Research-level optical microscope with advanced techniques
- Powerful software enables mapping of lines, areas, depth profiles, and cross-sectional slices
- Compact footprint
- Up to 2 lasers configurations
- Edge or Bragg filters
- Fully automated change of lasers, gratings with no additional system alignment
- High-resolution color video camera for a sample observation
- Research grade optical microscope
- Raman optical module
- Automated 3-position turret with mirrors for input/output radiation
- Confocal laser microscope module (“Reflection” module)
- Two-channel imaging spectrometer
- Z piezo scanner
- Manual or automated microscope stage
Click here for the Ramos S120 page on the Ostec Website.
RAMOS CARS is the only commercially available anti-stokes Raman instrument. This Multifunctional Coherent Anti-Stokes Raman Spectroscopy system (CARS) combines a CARS scanning microscope, a Raman / luminescent scanning confocal microscope, and a conventional scanning confocal laser microscope
Multi-channel – five channels for simultaneous high-speed measurements:
- E-CARS & Raman
- reflected laser radiation
- transmitted laser radiation
- luminescent laser radiation
Advantages of CARS method
- high sensitivity: CARS generates more intensive and directed signals in comparison to spontaneous Raman microscopy;
- anti-Stokes CARS signal has frequency exceeding pumping waves frequencies and is detected in a spectral range free from the stray light of Stokes luminescence;
- CARS signal is registrated only in a focus where excitation intensity is the highest. It allows imaging with a high spatial resolution using non-confocal pinholes and also performs 3D layer-by-layer scanning with minimal neighboring layers influence on measuring results;
- spectral resolution of CARS signal is defined only by the width of pumping lasers lines, what simplifies spectral measurements, as detection of CARS signals can be performed without any spectral instrument;
- CARS signal is proportional to the squared molecule concentration, it allows using CARS (along with the selectivity and noninvasivity of the method) for quantitative measurements of chemical substance concentration in a sample;
- minimal invasive (nondestructive) CARS method for biological samples. Due to the high sensitivity of CARS method molecules in living cells can be detected without fluorescent markers.
Click here for the RAMOS CARS page on the Ostec Website.
RAMOS E/M Series
3D scanning laser Raman microscopes provide rapid, high sensitivity analysis and unprecedented convenience in use
RAMOS E/M Series Raman spectrometers are designed on the basis of research grade optical microscopes allowing the realization of the following light microscopy methods:
- Raman measurements
- Transmitted light
- Reflected light (bright field and dark field illumination)
- Confocal microscopy
- Fluorescence measurements
- Polarization contrast and phase contrast imaging
- Differential interference contrast
The innovative approach to system design of Raman spectrometers ensures extremely high temperature and temporal stability of spectral measurements.
All components of RAMOS E200 system are fully integrated within an optical microscope providing compactness and mobility of the system.
In RAMOS M350, M520, M750 systems external imaging spectrographs are connected via optical fibers.
Raman measurements with the RAMOS E/M Series systems can be started in several minutes by turning a system key.
Click here for the RAMOS E/M Series page on the Ostec Website.
3D Scanning Laser Raman Microscope
The highest spectral and imaging resolution with specially designed spectrometer
Specially designed imaging spectrometer incorporates many features that make it ideal for confocal Raman measurements. The image of pinhole is projected to a multichannel detector without any aberrations.
- The smaller amount of illuminated pixels on the CCD matrix leads to the smaller dark counts and the higher spectral resolution.
- Spectral resolution of RAMOS N500 with an Echelle grating is 0.25cm-1
- Spectral image of the pinhole on the CCD camera (aberration free). CCD pixel size is 12 μm.
3D scanning laser confocal Raman microscope RAMOS N500 provides the acquisition of two images within a single scan: a Rayleigh image (using laser light reflected from a sample) and a spectral image by Raman scattering.
Ultrafast imaging option allows to get confocal image in 3 sec (3 μs/pixel).
RAMOS N500 uses fast beam scanning by galvano mirrors.
Layout of galvano mirror scanner module allows mapping with no intensity losses from image center to its edges.
Click here for the RAMOS N500 page on the Ostec Website.
Visit the Ostec Website.